Journal:

Article Title: Spermidine but not spermine is essential for hypusine biosynthesis and growth in Saccharomyces cerevisiae : Spermine is converted to spermidine in vivo by the FMS1 -amine oxidase

doi: 10.1073/pnas.1835918100

Figure Lengend Snippet: Yeast strains and plasmids

Article Snippet: This PCR product and Bam HI– Bst EII-digested vector DNA (pYES2.1-P GAL1 /V5-His/ lac Z, Invitrogen) were used to transform Y537 ( spe3 Δ FMS1 + ) and Y547 ( SPE3 + fms1 Δ) cells ( ) by using the lithium acetate method ( 27 ).

Techniques:

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Angiopoietin-1 prevents severe bleeding complications induced by heparin-like drugs and fibroblast growth factor-2 in mice

doi: 10.1152/ajpheart.00373.2015

Figure Lengend Snippet: PPS did not affect the expression of metalloproteinases or VEGF-A in the intestine of mice infected with rAd-LacZ. A: representative pictures of the Western blots done with samples derived from the small intestinal of mice infected with rAd-LacZ vectors and treated with PPS (60 mg/kg) or phosphate-buffered saline (PBS). Only the intestinal expression of ICAM-1 was significantly increased in mice treated with rAd-LacZ + PPS relative to the control mice infected with rAd-LacZ (*P < 0.05; n = 6 samples per group). B: the densitometric analysis corresponding to the Western blots. Results are expressed in arbitrary optical density units as ratio of the beta-actin expression (means ± SE). Samples from 6 different mice were included in each group.

Article Snippet: rAd-vectors carrying either the Escherichia coli LacZ gene (rAd-Lac-Z), a 700-bp cDNA sequence encoding a secreted form of human recombinant FGF-2 (rAd-FGF-2), or a genetically modified form of angiopoietin-1 (Ang-1*) from Regeneron Pharm (Tarrytown, NY), were generated, amplified, and purified as previously described ( 13 , 21 , 44 , 50 ).

Techniques: Expressing, Infection, Western Blot, Derivative Assay, Saline, Control

Journal: American Journal of Physiology - Heart and Circulatory Physiology

Article Title: Angiopoietin-1 prevents severe bleeding complications induced by heparin-like drugs and fibroblast growth factor-2 in mice

doi: 10.1152/ajpheart.00373.2015

Figure Lengend Snippet: PPS did not affect the expression of metalloproteinases or VEGF-A in the intestine of mice infected with rAd-LacZ. A: representative pictures of the Western blots done with samples derived from the small intestinal of mice infected with rAd-LacZ vectors and treated with PPS (60 mg/kg) or phosphate-buffered saline (PBS). Only the intestinal expression of ICAM-1 was significantly increased in mice treated with rAd-LacZ + PPS relative to the control mice infected with rAd-LacZ (*P < 0.05; n = 6 samples per group). B: the densitometric analysis corresponding to the Western blots. Results are expressed in arbitrary optical density units as ratio of the beta-actin expression (means ± SE). Samples from 6 different mice were included in each group.

Article Snippet: Recombinant adenoviral (rAd) vectors. rAd-vectors carrying either the Escherichia coli LacZ gene (rAd-Lac-Z), a 700-bp cDNA sequence encoding a secreted form of human recombinant FGF-2 (rAd-FGF-2), or a genetically modified form of angiopoietin-1 (Ang-1*) from Regeneron Pharm (Tarrytown, NY), were generated, amplified, and purified as previously described ( 13 , 21 , 44 , 50 ).

Techniques: Expressing, Infection, Western Blot, Derivative Assay, Saline, Control

Journal: PLoS Biology

Article Title: A Lack of Premature Termination Codon Read-Through Efficacy of PTC124 (Ataluren) in a Diverse Array of Reporter Assays

doi: 10.1371/journal.pbio.1001593

Figure Lengend Snippet: (A) Incubation with G418 for 24 hours leads to a dose-dependent increase in enzyme activity of stably transfected PTC-β-Gal. (B) 24-hour incubation with PTC124 at concentrations in excess of its reported effective concentration range of 0.1–30 µM has no effect. All data points represent mean ± standard deviation (n = 5).

Article Snippet: The cysteine 320 codon of the lac Z gene in the pSV-β-galactosidase vector (Promega) was replaced by a premature TGA stop codon using the QuickChange site-directed mutagenesis system (Stratagene) with the following primers: pSV- lac Z-TGA.F 5′ GAT TGA AGC AGA AGC ATG AGA TGT CGG TTT CCG CG 3′ and pSV- lac Z-TGA.R 5′ CGC GGA AAC CGA CAT CGC AGG CTT CTG CTT CAA TC 3′ .

Techniques: Incubation, Activity Assay, Stable Transfection, Transfection, Concentration Assay, Standard Deviation